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At each time point, 20 L of iHLO culture medium was transferred to a 384-well white flat-bottom plate, 20 L of Luciferin Detection Reagent was added, and the mixture was incubated for 30 min before measuring luminescence

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Anti-glycation effect Glycation is the process by which sugar (glucose, fructose) reacts non-enzymatically with proteins to form Advanced Glycation End-products (AGEs)